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FerroSelect™ QP negative T cell selection Kit

“Negative” selection resulting in untouched T cells using the FerroSelect™ Quadrupole Magnets.

“Negative” selection resulting in untouched T cells using the FerroSelect™ Quadrupole Magnets.

Product Dimensions

L x W x D: 98.43 x 43.66 x 62.71 mm

Histograms

Histograms from a CD3+ cell selection performed by the depletion of other cell types using Biomagnetic Solutions single depletion reagent (biotinylated) along with streptavidin ferrofluid and the QP5 Quadrupole magnet.

Pre-Separation

Post-Separation

Scaleability of the Ferroselect Quadrupole Cell Selection System

Enrichment of CD3 Cells by Removing Other Cell Populations (Negative Depletion)

The depletion of other cell types from leukopaks creates a highly enriched T cell population that has no capture reagents on their surface. This is accomplished through the use of a single targeting monoclonal antibody. Cells from leukopaks were washed by centrifugation and routinely resuspended at 1.0 x 108 cells/ml. Aliquots of cells were incubated with a set concentration of the “depleting” biotinylated monoclonal antibody and streptavidin ferrofluid. Cells were adjusted to a final concentration of 2.0 x 107 cells/ml and 4.0 ml of cell suspension was separated in the smaller FerroSelect QP5 Quadrupole and 12.5ml of cells separated in the larger QP15 magnet. The supernatants containing cells not captured by the magnetic field, i.e the cells of interest, were gently drained from the tube, counted and subjected to FACS analysis. Each experiment was undertaken three time with different starting products

  • No need to wash out excess monoclonal antibody, reducing processing time and cell loss through washing
  • Average purity > 85% using the FerroSelect QP5
  • Average yield > 60% using the FerroSelect QP5
  • Designed for multiple separations using the FerroSelect Quadrupoles
  • No need to wash apheresis product prior to incubation

Intended Use

Small-scale cell selections using the FerroSelect Quadrupole Magnet

Product Restrictions

RUO, ex vivo use only

Additional Resources

26-0005-01 QP5 Negative Selection Insert | Download

26-0044-01 QP15 Negative Selection Insert | Download