BioMagnetic Solutions’ product offering consists of streptavidin coated ferrofluids, biotinylated antibodies, and cell separation devices, some requiring our own disposables. These support our vision of allowing you to move from the research bench to the clinic using the same reagents and processing conditions.
Highlights: Convenience, Time & Cost Savings
Our ferrofluids are manufactured by coupling recombinant human serum albumin (HSA) onto crystalline cores of magnetite. Streptavidin is subsequently covalently linked to the HSA. These ferrofluids are highly magnetic with a large surface area and their unique size allows them to be both sterile filtered and used with open field magnets and biotinylated monoclonal antibodies to elicit a cell separation. Manufactured currently, for research use only, clinical products are also set to be produced once our new cGMP facility is commissioned.
Cell Selection Application
We offer FerroSelect reagent kits that allow both the positive selection and negative depletion of cells. Each kit currently contains a single monoclonal antibody and a vial of streptavidin ferrofluid. These are specifically designed to work with the cBioMagnetic Solutions’ cell selection devices. Talk with our customer support team in determining the best fit for your needs!
The anti-CD3 positive selection kit allows for the rapid selection of T-cells from peripheral blood mononuclear cells (PBMC) or similar products. Following a brief incubation with the mAb streptavidin ferrofluid is added without the need to wash out excess mAb. A highly enriched T cell product can be produced by capturing “magnetically” coated cells using either the BioMagnetic Solutions Quadrupole or Ferroselect magnetic arrays.
We also provide a kit that allows for the expansion of the enriched T cells. This kit contains two antibodies, the biotinylated anti-CD3 for cell selection and a biotinylated anti-CD28 both driving T-cell expansion and activation. Once the T-cell selection procedure is completed the biotinylated anti-CD28 mAb is simply added to the cells along with Interleukin-2 to bring about expansion and cell activation. Control of the activation state of the cells is in the hands of the researcher as biotinylated anti-CD28 can be titrated into the system independently of biotinylated anti-CD3
Kits are offered in multiple versions based upon the size of the starting cell product and are available in either single or five packs. These are currently for research use only, with “clinical” versions on their way.
Our scientists have developed a unique approach to “negative cell depletion” that can be achieved using a single biotinylated monoclonal antibody and streptavidin ferrofluid. Furthermore, the enrichment of T cells is possible without washing the starting PBMC product. Following incubation with this biotinylated monoclonal antibody, streptavidin ferrofluid is added to the cellular milieu without the need to remove excess mAb. Cells can then be separated either using the BioMagnetic Solutions quadrupole devices or their FerroSelect planar array of magnets.
Kits are available in different sizes depending upon the number of cells to be separated. They can be purchased either as individual units or in packs of five. Currently they are intended for research use only,although a “clinical” version is currently under development.
From the Bench to the Clinic
Our unique solutions allow researchers to drive their studies from the research bench to the clinic. We offer an array of products that work in concert to resolve your cell selection needs. Quadrupole magnets allow you to work at small scale and then move to the Ferroselect planar arrays that permit cell selection without the use of columns. Although we offer research use only kits currently, clinical products will be available when our cGMP facilities are open.