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FerroSelect™ QP anti-CD3 Kit

“Positive” selection of CD3+ T cells using the FerroSelect™ Quadrupole Magnets.

“Positive” selection of CD3+ T cells using the FerroSelect™ Quadrupole Magnets.

Product Dimensions

L x W x D: 98.43 x 43.66 x 62.71 mm

Histograms

Histograms from a CD3+ cell selection performed with biotinylated anti-CD3 and streptavidin ferrofluid using the QP5 Quadrupole magnet.

Pre-Separation

Post-Separation

Scaleability of the Ferroselect Quadrupole Cell Selection System

CD3 Positive Cell Selection

Cells from leukopaks were washed by centrifugation and resuspended at 1.0 x 108 cells/ml. Aliquots of cells were incubated with a set concentration of biotinylated anti-CD3 monoclonal antibody and streptavidin ferrofluid and adjusted to a final concentration of 2.0 x 107 cells/ml. Four millilitres of cell suspension was separated in the smaller FerroSelect QP5 Quadrupole and 12.5ml of cells separated in the larger QP15 magnet.

The supernatants containing cells not captured by the magnetic field were gently drained from the tube, the tube removed from the magnet and the cells resuspend in fresh medium. The cells were recaptured in the respective Quadrupole magnets, the supernatants drained and residual cells resuspended for cell counting and FACS analysis. Each experiment was undertaken three time with different starting products.

  • No need to wash out excess anti-CD3, reducing processing time and cell loss through washing
  • Average purity > 90% using the FerroSelect QP5
  • Average yield > 50% using the FerroSelect QP5
  • Designed for multiple separations using the FerroSelect Quadrupoles

Intended Use

Small-scale cell selections using the FerroSelect Quadrupole Magnets

Product Restrictions

RUO, ex vivo use only

Additional Resources

26-0002-01 QP5 Positive Selection Insert | Download

26-0043-01 QP15 Positive Selection Insert | Download