Products // Reagent Kits
FerroSelect™ CD3/CD28 Activation/Expansion Kit for either the selection and/or activation of T cells
FerroSelect™ CD3/CD28 Activation/Expansion Kit for either the selection and/or activation of T cells
Tunable T cell activation & expansion in a single step
Expansion of T cells requires a stimulatory event, typically triggered ex vivo by activating cells via their CD3 and CD28 receptors. Clustering of T cell receptors is accomplished by complexing our FerroSelect ferrofluids together with biotinylated anti-CD3 and anti-CD28 antibodies. Artificial cross-linking of the receptors and/or three-dimensional changes of the receptor complex initiates a signaling cascade driving cells into division and concomitant cytokine release. Artificially adding Interleukin-2 (IL-2) further drives T cells into activation and division.
Expansion of T cells following their selection using the FerroSelect CD3+ Kit. T cells were seeded at 5 x 105 cells per ml in the presence of 1.5 ug/ml of anti-CD28 and 10.0 ng/ml of IL-2.
Expansion of T cells following their enrichment by depleting unwanted cells using the AbsenT CD3+ Kit. Cells were seeded at 5 x 105 cells per ml in the presence of 1.5 ug/ml of anti-CD28, 1.5 ug/ml of anti-CD3, 10.0 ug/ml of streptavidin ferrofluid and 10.0ng/ml of IL-2.
Maintenance of the naive state of T cells following their activation and expansion. T cells were enriched using the AbsenT kit and expanded using the conditions detailed above. The naive status of the T cells over the period of activation/expansion was determined by the surrogate markers CD45Ra and CCR7.
Low level PD-1 expression on cells cultured with biotinylated anti-CD3, anti-CD28 and streptavidin ferrofluid on day15 of culture. Low levels of the surrogate marker are indicative of their ability to activate in the presence of appropriate stimuli.
