BioMagnetic Solutions’ patent-pending SIAT™ protocol allows T cells to be Simultaneously Isolated and Activated to permit downstream transduction and expansion of CAR-T cells. Employing the common-capture Ferrofluids, isolation and activation of T cells from leukapheresis products in 1-Step has been demonstrated. Such T cells have been successfully transfected with a GFP plasmid with high efficiency and viability resulting in cell expansions of 600-fold over 18 days.