Positively select, or deplete, CD3+ cells from apheresis or PBMC. Following consecutive incubations with the antibody and Ferrofluid, the positively-selected cells are removed using the XpresSep™ Multipole Magnetic Separator.
The biocompatibility of the Ferrofluid allows for immediate use of the selected cells in downstream studies as cell viability and biological function are unaffected by the separation.
Figure 1. XpresSep™ Human CD3 Positive Selection Kit
Beginning with peripheral blood mononuclear cells (PBMCs), the CD3+ (T cell) percentage of the positively selected cells is typically > 95%.