Obtain untouched CD3+ cells from apheresis or PBMC. Following consecutive incubations with the antibody cocktail and Ferrofluid, unwanted cells are removed using the XpresSep™ Multipole Magnetic Separator.
Figure 1. XpresSep™ Human CD3 Negative Selection Kit
Beginning with peripheral blood mononuclear cells (PBMCs), the CD3+ (T cell) percentage of the negatively selected cells is typically > 95%.